For any laboratory adopting bead-based multiplex assays, investing time in learning the nuances of their FCAP software—gating strategies, curve fitting parameters, and QC metrics—is as critical as pipetting accurately. The software does not replace scientific judgment; it amplifies it.
Quantification is impossible without a standard curve. The software ingests data from a series of standards with known analyte concentrations (typically 7-10 points, serially diluted). FCAP software applies either a or 5-Parameter Logistic (5-PL) regression model. These models are superior to linear regression because they account for the sigmoidal shape of immunoassay response—asymptotic at low and high concentrations. The software automatically flags outliers, calculates R² values, and provides confidence intervals. A well-constructed standard curve (R² > 0.99) is the hallmark of a valid run. fcap array software
Raw data is useless if it is trapped in a proprietary format. Leading FCAP software exports to: The software ingests data from a series of
The software's primary role is to manage microbead-based multiplex assays. By utilizing the fluorescence intensities of different bead populations, it can simultaneously identify and quantify multiple analytes within a single sample—up to 72 different analytes per sample in some versions. The software automatically flags outliers