| Parameter | Report as | |-----------|------------| | EC50 | 1.2e-7 M (95% CI: 8.5e-8 to 1.7e-7) | | LogEC50 | -6.92 ± 0.08 | | Hill slope | 1.2 ± 0.15 | | Top | 98.3% ± 2.1 | | Bottom | 4.2% ± 1.3 |

: The calculator supports units from femtomolar to molar , and Prism allows you to easily transform these values (such as converting to log scale) for non-linear regression analysis.

: Prism handles scientific notation ( 1e-9 = 1 nM). Use consistent units (e.g., all in M).

: Calculate the final volume required to achieve a target concentration based on the mass you have on hand.

: By plotting response against molar concentration, Prism can automatically calculate potency values, such as the EC50 (the concentration that provokes a half-maximal response). Pro Lab Tips Molarity Calculator - GraphPad